Composite

Part:BBa_K2066001

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-07-12)
Revision as of 21:02, 12 July 2016 by Registry (Talk | contribs)

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UNS Standard Adapter with Red Chromoprotein.

This part serves as an adapter to the UNS standard adopted by the WM iGEM 2016 team. The part expresses a red chromoprotein as an indicator molecule and teams can perform PCR to amplify the backbone using a UNS 2 reverse and UNS 3 forward primer. This will allow you to go into a gibson assembly with another fragment containing USN 2 and 3 at either end.

The UNS2 and UNS3 sequences are taken from: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None