Measurement

Part:BBa_J100279:Design

Designed by: Anna Buser   Group: Campbell M Lab   (2016-06-24)
Revision as of 14:47, 6 July 2016 by Anbuser (Talk | contribs) (→‎Design Notes)

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tClone Tet+PDB riboswitch with P9 and BD10


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 350
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 496
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 522
    Illegal NgoMIV site found at 890
    Illegal NgoMIV site found at 1050
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since there was only a 6 base difference between BD18 C Dog and BD10 C Dog, the PCR was used to insert in and amplify the new BD10 C Dog RBS rather than during GGA. GGA was still necessary to make the dsDNA into a plasmid.

Note that the PDB riboswitch's last base is a C instead of a T, which may help this riboswitch to be less leaky.

Source

J100275

References