Coding

Part:BBa_K2010004:Design

Designed by: William Cho   Group: iGEM16_Harvard_BioDesign   (2016-06-21)
Revision as of 17:55, 21 June 2016 by Registry (Talk | contribs)

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sfGFP + PETase (PET-degrading enzyme, origin I. sakaiensis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 743
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1161


Design Notes

The DNA sequence has been optimized for E. coli K12 and modified to facilitate gBlock synthesis, by avoiding repeated sequences.



Source

This sequence is publicly known and was privately modified.

References