Coding

Part:BBa_K2010003

Designed by: William Cho   Group: iGEM16_Harvard_BioDesign   (2016-06-21)
Revision as of 17:50, 21 June 2016 by Registry (Talk | contribs)

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YebF + PETase (PET-degrading enzyme, origin I. sakaiensis)

This GGS linker is a linker sequence that neutrally connects two protein domains together. The linker is composed of repetitions of glycine-glycine-serine, which are known to be neutral and not interfere with the rest of the protein. This is an alternate version of BBa_K2010002, but serves the same function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 280
    Illegal SapI site found at 816


[edit]
Categories
//function/degradation
Parameters
protein