Coding

Part:BBa_K2010000:Design

Designed by: William Cho   Group: iGEM16_Harvard_BioDesign   (2016-06-17)
Revision as of 18:12, 17 June 2016 by Williamcho (Talk | contribs)


PETase (PET-degrading enzyme, origin I. sakaiensis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 426


Design Notes

This sequence was optimized for E. coli K12 using the Codon Optimization Tool from the IDT website.


Source

This part, originally named ISF6_4831, comes from Ideonella sakaiensis, identified in "A bacterium that degrades and assimilates poly(ethylene terephthalate)" (Yoshida et al. 2016).

References