Plasmid

Part:BBa_J100270

Designed by: Owen Koucky   Group: Campbell M Lab   (2016-06-17)
Revision as of 16:11, 17 June 2016 by Registry (Talk | contribs)

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SP-T7Specific_L2-48.3 (Carlson et al. 2014)

In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100270 to be used as a selection plasmid in phage-assisted continuous evolution (PACE).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5475
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2016
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5475
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5475
    Illegal NgoMIV site found at 8437
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None