Plasmid

Part:BBa_J100266

Designed by: Owen Koucky   Group: Campbell M Lab   (2016-06-17)
Revision as of 14:30, 17 June 2016 by Registry (Talk | contribs)

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pJC173g-SD8_AP-neg (Carlson et al. 2014)

In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100266 to be used as an accessory plasmid in phage-assisted continuous evolution (PACE).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1292
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1292
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1991
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1292
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1292
    Illegal NgoMIV site found at 3352
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 981
    Illegal BsaI.rc site found at 3060
    Illegal SapI site found at 2158
    Illegal SapI.rc site found at 790


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