Intermediate

Part:BBa_K1904005

Designed by: Enrique Chimal Juarez, Roger Milton Rubio Sánchez, Enrique Arturo Salas Palomino, Brian Morteo Luna   Group: iGEM16_TecCEM   (2016-06-07)
Revision as of 03:48, 8 June 2016 by RRUBIO (Talk | contribs)

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Fused protein (Holin/Endolysin) with RBS and terminator, C-terminal HisTag, (RFC 25 N-Part).

This part contains the coding sequences of BBa_K1904002 and BBa_K1904004 which consist of an specific endolysin for Acinetobacter baumannii and a T4 holin, forming a fusion protein. Additionally the part is assembled with B0034 (RBS) and B0015 (double terminator). This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage. The standard of the biobrick is RFC 25, but as it does not tolerate changes in the N-part of the protein it is designed as an Freiburg N-part. Ultimately, a C-terminal HisTag was added in order to allow its purification via a Nickel-affinity chromatography.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None