Intermediate

Part:BBa_K1904003

Designed by: Enrique Chimal Juarez, Roger Milton Rubio Sánchez, Enrique Palomino, Brian Morteo   Group: iGEM16_TecCEM   (2016-06-07)
Revision as of 03:41, 8 June 2016 by RRUBIO (Talk | contribs)

T4 Holin with RBS and terminator.

This biobrick contains the coding sequence for a T4 Holin (BBa_K112805) which promotes pore formation on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. The part, designed in RFC10, was assembled with B0034 (RBS) and B0015 (double terminator). This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None