Intermediate
Part:BBa_K1904005
Designed by: Enrique Chimal Juarez, Roger Milton Rubio Sánchez, Enrique Arturo Salas Palomino, Brian Morteo Luna Group: iGEM16_TecCEM (2016-06-07)
Fused protein (Holin/Endolysin) with RBS and terminator, C-terminal HisTag, (RFC 25 N-Part).
This part contains the coding sequences of BBa_K1904002 and BBa_K1904004 which consist of an specific endolysin for Acinetobacter baumannii and a T4 holin, forming a fusion protein. Additionally the part is assembled with B0034 (RBS) and B0015 (double terminator). This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage. The standard of the biobrick is RFC 25, but as it does not tolerate changes in the N-part of the protein it is designed as an Freiburg N-part. Ultimately a C-terminal HisTag was added in order to allow its purification via a Nickel-affinity chromatography.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |