Intermediate

Part:BBa_K1904003

Designed by: Enrique Chimal Juarez, Roger Milton Rubio Sánchez, Enrique Palomino, Brian Morteo   Group: iGEM16_TecCEM   (2016-06-07)
Revision as of 20:46, 7 June 2016 by Registry (Talk | contribs)

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T4 Holin with RBS and terminator.

This biobrick is contains the coding sequence for a T4 Holin (BBa_K112805) which form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. The part is assembled with B0034 (RBS) and a double terminator (B0015). This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None