Part:BBa_K1713004

Plac/ara-1+strong RBS+mRFP+double terminator

The hybrid promoter ( Plac/ara-1 ) can be activated by the AraC protein in the presence of arabinose and so that mRFP produced. And then the LacI protein would repress this hybrid promoter ( Plac/ara-1 ) in the absence of isopropyl b-D-1-thiogalactopyranoside (IPTG).

Attention: After sequencing, we found that this plasmids have two sites error on Suffix which is after the end of hybrid promoter sequence "cacaca" and the initial two bases of Suffix is changed from T-A to GAA. We can not make sure whether it will have an impact on function till now.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 28
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 683
  Illegal AgeI site found at 795
  
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

This device is composed with the hybrid promoter(Plac/ara-1) and a mRFP linked behind it. Some related tests are specifically run under two different chemical inducers(Arabinose/IPTG) respectively. A series of different inducer's concentrations are set to test amples, and the expressions level of mRFP is linked with the influence that changed concentration of inducer had on the promoter. Related data after analysis show as the following charts.

Fig 1:The effect on the promoter of Arabinose. Fig 2:The effect on the promoter of IPTG.

Based on results above, this device reveals to be activated by the AraC protein in the presence of arabinose and repressed in the absence of isopropyl b-D-1-thiogalactopyranoside (IPTG).