Plasmid

Part:BBa_M36533

Designed by: Inderpreet Kaur Hayer   Group: Stanford BIOE44 - S11   (2015-10-24)
Revision as of 06:47, 8 December 2015 by Htale1 (Talk | contribs)

Arsenate Bioremediation Actuator Plasmid Containing PHO84 Gene

This part was made in order to reduce arsenate to arsenite and increase arsenite resistance in S. cerevisiae.

Construct uses DNA 2.0 plasmid backbone (pD1207) with GAL1 inducible (galactose-inducible/glucose-repressible) promoter, high copy number ORI, and ampicillin and histidine selectable markers in E. coli and yeast, respectively. A self-cleaving 2A peptide sequence was inserted between ACR2 and ACR1 genes to allow for both genes to be transcribed.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1647
    Illegal PstI site found at 1002
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1002
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1438
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1647
    Illegal PstI site found at 1002
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1647
    Illegal PstI site found at 1002
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None