Tag

Part:BBa_M36144:Experience

Designed by: Tatyanna Dadabbo & Richard Hojel & Soraya Karimi   Group: Stanford BIOE44 - S11   (2015-10-22)
Revision as of 07:03, 5 December 2015 by Tdadabbo (Talk | contribs) (→‎User Reviews)

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Applications of BBa_M36144

User Reviews

UNIQf9781c10d22df6f9-partinfo-00000000-QINU UNIQf9781c10d22df6f9-partinfo-00000001-QINU

12/04/15

This His tag was codon optimized for E.Coli and specific for the EP-B2 protein. It's a marker for the EP-B2 protein that can be used for identification. Another purpose of the His tag is protein purification, specifically The EP-B2 protein. When planning our experiments, we were going to do a Western Blot, however, we ended up not needing to do a Western Blot, because our other assays provided sufficient results. Since we did not do a Western Blot the His tag was not actually a necessary part of the construct. If we end up doing follow-up experiments this His tag will be necessary.