Tag

Part:BBa_M36144:Experience

Designed by: Tatyanna Dadabbo & Richard Hojel & Soraya Karimi   Group: Stanford BIOE44 - S11   (2015-10-22)
Revision as of 06:01, 5 December 2015 by Tdadabbo (Talk | contribs)

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Please enter how you used this part and how it worked out.

Applications of BBa_M36144

User Reviews

UNIQ77933763426e5d1e-partinfo-00000000-QINU UNIQ77933763426e5d1e-partinfo-00000001-QINU

12/04/15

This His tag was codon optimized for E.Coli and worked properly with both our constructs, one with mature EP-B2 transformed E.Coli cells and one with Pro Ep-B2 transformed E.Coli cells. The His tag was successful in purifying the targeted protein (mature EP-B2 or Pro EP-B2). We had lots of fluorescence increase in the lysed cells assay for both the mature EP-B2 and Pro EP-B2, which shows a large amount of the protein was present, and having it purified amplified these results.