Coding

Part:BBa_M36402:Experience

Designed by: Walter Roper, Theresa Sievert, Connor Gonzalez   Group: Stanford BIOE44 - S11   (2015-10-25)
Revision as of 23:59, 3 December 2015 by Wroper (Talk | contribs)

Stanford Location

Plasmid Name: WTC_DNA2

DNA 2.0 Gene#: 231900

Organism: E.coli

Device Type: Actuator

Barcode # of Glycerol Stock: 133021775

Box Label: BioE44 S15 Box 2

Applications of BBa_M36402

This part has not been used in any other composite parts.

Performance Data of BBa_M36402

In order to assay if this plasmid design successfully created the ester methyl salicylate in the presence of salicylic acid we created tubes of transfected E.coli with varying concentration of salicyclic acid and ran these tubes is a GC-MS machine to see if levels of the desired product, methyl salicylate, were detectable. the tubes contained .001mM, .01mM, .1mM, 1.M, and 10mM of salicylic acid. When comparing our plasmid GC-MS readings with prior reading we had gained by testing pure salicylic acid we discovered that this plasmid design did not produce any detectable levels of methyl salicylate and conclusively was an ineffective part.

User Reviews

UNIQ1dbb9a9dde205a6d-partinfo-00000000-QINU UNIQ1dbb9a9dde205a6d-partinfo-00000001-QINU