Coding
Part:BBa_K1728021
Designed by: Wan-Yun Chiu, Ming-Fei Hsieh Group: iGEM15_CGU_Taiwan (2015-09-17)
Chemokine receptor 1 (CXCR1)
Chemokine receptor 1 (CXCR1) CXCR1 (also named with IL8RA and CD181) has high affinity in binding with IL8. CXCR1 belongs to the GPCR rhodopsin-like family and has seven-transmembrane domains, with the N-terminus exposed to the extracellular space and the C-terminus exposed to cytoplasm. Different from ordinary GPCR ligand binding sites, in which of CXCR1 locate on N-terminal and extracellular loop 3 of CXCR1. To improve heterologous expression of CXCR1 in yeast, we inserted two different signal peptide sequences upstream of the CXCR1 sequence. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 786
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 211
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 975
[edit]
Categories
Parameters
//chassis/eukaryote/yeast
None |