Plasmid

Part:BBa_M36534:Design

Designed by: Inderpreet Kaur Hayer   Group: Stanford BIOE44 - S11   (2015-10-24)
Revision as of 20:25, 26 October 2015 by Htale1 (Talk | contribs) (→‎Design Notes)


Arsenate Bioremediation Actuator Plasmid Containing ACR 1 and ACR 2 Genes


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 873
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 873
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 873
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 873
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 873
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ACR 2 was ordered first due its increased importance vs. ACR 1.


http://imageshack.com/a/img907/254/OL6vcf.png

Source

DNA 2.0 for general plasmid design[1] including restriction sites, GAL1 inducible promoter, high copy number, and sequence for ACR 1 and 2 from yeastgenome website [http://www.yeastgenome.org/locus/S000006403/sequence] [http://www.yeastgenome.org/locus/S000006404/sequence]

RBS consensus yeast Kozak sequence taken from the Silver Lab, BBa_J63003[2] was used.

References