Coding

Part:BBa_M36402:Design

Designed by: Walter Roper, Theresa Sievert, Connor Gonzalez   Group: Stanford BIOE44 - S11   (2015-10-25)
Revision as of 06:16, 25 October 2015 by Wroper (Talk | contribs) (→‎References)


Salicylic Acid to Methyl Salicylate Actuator #2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 652
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 232


Design Notes

We made sure to remove different restriction sites that would interfere with the creation or function of the enzyme, namely BsaI and SapI which is used by DNA 2.0 for Electra cloning.


Source

This part is derived from SAMT from Clarkia breweri.

References

Part:BBa_J45005, who created the actual part that we adapted to fit our project.