Coding

Part:BBa_M36401:Design

Designed by: Walter Roper, Theresa Sievert, Connor Gonzalez   Group: Stanford BIOE44 - S11   (2015-10-25)
Revision as of 06:15, 25 October 2015 by Wroper (Talk | contribs) (→‎References)

Salicylic Acid to Methyl Salicylate Actuator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 213
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 213
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 213
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 213
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A design considerations we had was the optimization of codons.We condon optimized this particular DNA sequence using IDT's Codon Optimzation Tool by changing its codons to match the most prevalent tRNAs. This way the protein will be more efficiently translated and make more of the desired product. This is the only relevant design change to this part.

Source

This part is derived from SAMT from Antirrhinus majus.

References

Part:BBa_J45001, who created the actual part that we adapted to fit our project.