Plasmid

Part:BBa_M36534

Designed by: Inderpreet Kaur Hayer   Group: Stanford BIOE44 - S11   (2015-10-24)
Revision as of 02:43, 25 October 2015 by Htale1 (Talk | contribs)

Arsenate Bioremediation Actuator Plasmid Containing ACR 1 and ACR 2 Genes

This part was made in order to enhance arsenic absorption and resistance in S. cerevisae.

DNA 2.0 Plasmid (pD1204) with GAL1 inducible promoter, high copy number, and leucine as the selectable marker


Part contains (in order): RBS: consensus yeast Kozak Sequence

Spacer sequence

ACR2 gene

Stop codon

Spacer sequence

RBS: consensus yeast Kozak Sequence

Spacer sequence

ACR1 gene

Stop codon


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 873
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 873
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 873
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 873
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 873
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None