Device

Part:BBa_M36532:Design

Designed by: Inderpreet Kaur Hayer   Group: Stanford BIOE44 - S11   (2015-10-24)
Revision as of 01:14, 25 October 2015 by Htale1 (Talk | contribs) (→‎Design Notes)


Arsenate Bioremediation Actuator in S. Cerevisiae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2711
    Illegal XbaI site found at 1680
    Illegal PstI site found at 1035
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2711
    Illegal PstI site found at 1035
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2711
    Illegal BglII site found at 1471
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2711
    Illegal XbaI site found at 1680
    Illegal PstI site found at 1035
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2711
    Illegal XbaI site found at 1680
    Illegal PstI site found at 1035
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2184


Design Notes

Below is an example of a possible implementation of BBa_M36532, the Arsenate Bioremediation Actuator. In this example, a GAL1 inducible sensor has been attached upstream of the actuator. This image shows the different components of the Arsenate Bioremediation Actuator. Part numbers for the Kozak sequence and the PHO84, ARC1 and 2 genes can be found in the following section.


Example implementation:












Due to the size of PHO84, it was necessary to design a separate construct with the ACR genes, 1 and 2.

Source

Naturally occurring genes in S. cerevisiae: Pho84, ACR 1 and 2

References