Plasmid

Part:BBa_M36534

Designed by: Inderpreet Kaur Hayer   Group: Stanford BIOE44 - S11   (2015-10-24)
Revision as of 00:45, 25 October 2015 by Registry (Talk | contribs)

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Arsenate Bioremediation Actuator Plasmid Containing ACR 1 and ACR 2 Genes

This part was made in order to enhance arsenic absorption and resistance in S. cerevisae.

DNA 2.0 Plasmid (pD1204) with GAL1 inducible promoter, high copy number, and Uracil as the selectable marker Part contains (in order): RBS: consensus yeast Kozak Sequence Spacer sequence ACR2 gene Stop codon Spacer sequence RBS: consensus yeast Kozak Sequence Spacer sequence ACR1 gene Stop codon


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 873
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 873
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 873
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 873
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 873
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None