Protein_Domain

Part:BBa_M36142:Design

Designed by: Tatyanna Dadabbo & Richard Hojel & Soraya Karimi   Group: Stanford BIOE44 - S11   (2015-10-22)
Revision as of 22:59, 22 October 2015 by Tdadabbo (Talk | contribs) (→‎Design Notes)

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Mature Cysteine Proteinase EP-B2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 381
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 49
    Illegal AgeI site found at 133
    Illegal AgeI site found at 472
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mature version of EP-B2 protein found in barley that breaks down gliadin found in gluten. Testing whether mature EP-B2 is enough for cells to survive.

Source

DNA sequence for mature EP-B2 generated from amino acid sequence in "Computational Design of an α-Gliadin Peptidase".

References