Composite

Part:BBa_K1675009

Designed by: Hui Luo   Group: iGEM15_BIT-China   (2015-09-12)
Revision as of 23:29, 27 September 2015 by Biotar (Talk | contribs)

a device to produce alkali and neutralize the external acidic environment

This device is part of our basic regulation circuit in 2015 project of BIT-China.

Using an acid induced promoter asr(BBa_K1170000), strong RBS(BBa_B0034),alkaline substance producing gene gadA coding region(BBa_K1675003).Will produce alkali under acidic environment and neutralize the external pH.

Fermentation 1.png

Fig.1 The final fermentation result of the basic regulation system under acidic stress. By calculating the pH change of unit active biomass in unit time, This figure can be drawed.

Fermentation 2.png

Fig.2 The growth curve of E.coli with basic regulation circuit under acidic stress.

Fermentation 3.png

Fig.3 The final fermentation result of the basic regulation system under alkaline stress. By calculating the pH change of unit active biomass in unit time, This figure can be drawed.

Fermentation 4.png

Fig.4 The growth curve of E.coli with basic regulation circuit under alkaline stress.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 844
    Illegal AgeI site found at 904
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
biology