Composite

Part:BBa_K1689007:Design

Designed by: ZHANG Yihao   Group: iGEM15_Peking   (2015-09-16)
Revision as of 18:09, 27 September 2015 by Dawnec (Talk | contribs) (→‎Design Notes)

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dCas9-C-luc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1176
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3
    Illegal BamHI site found at 3455
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4457
    Illegal AgeI site found at 4596
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 4207


Design Notes

Golden Gate Assembly

Source

E.coli

References