![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K1741014
Designed by: Marta Żardecka Group: iGEM15_UAM_Poznan (2015-09-17)
Revision as of 08:21, 27 September 2015 by Michal.Kopa (Talk | contribs)
sfGFP under proD promoter
An isolated constitutive promoter proD (see Davis, 2010) exhibits higher expression rates than proC. Expression profiles of proC and proD are similar in different media. Their expression levels depend on E. coli growth rate, proving that expression driven by those promoters is constitutive.
Results
Legend:
proC [BBa_K1741012]
proC with a hairpin in 5'UTR [BBa_K1741013]
proD [BBa_K1741014]
The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.
We observed that proD is up to three times stronger than proC. The hairpin concept has to be further elaborated upon and improved so as to stop translation in lower temperatures.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 101
Illegal SapI.rc site found at 201
[edit]
Categories
Parameters
None |