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Generator
Part:BBa_K1741014
Designed by: Marta Żardecka Group: iGEM15_UAM_Poznan (2015-09-17)
Revision as of 08:11, 27 September 2015 by Michal.Kopa (Talk | contribs)
sfGFP under proD promoter
A strong insulated constitutive promoter proD (cf. Davis 2010) is stronger than proC. Expression profiles of proC and proD are similar on different media = both seem to be really constitutive and the expression level does depend on E.coli growth rate.
Results
Legend:
proC [BBa_K1741012]
proC with a hairpin in 5'UTR [BBa_K1741013]
proD [BBa_K1741014]
The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.
We observed that proD is up to three times stronger than proC. The hairpin concept has to be further elaborated upon and improved so as to stop translation in lower temperatures.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 101
Illegal SapI.rc site found at 201
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