Generator

Part:BBa_K1741013

Designed by: Marta Żardecka   Group: iGEM15_UAM_Poznan   (2015-09-17)
Revision as of 07:34, 27 September 2015 by MartaZ (Talk | contribs) (→‎Results)

sfGFP under proC promoter with a hairpin in 5'UTR

A weak hairpin introduced to 5’UTR slightly lowers transcription/translation. Will be extended to a thermometer, actually we see no temp. dependence.

UAMpoznanproChairpin.jpg

This is the potential hairpin sequence.


Results

Legend:

proC [BBa_K1741012]

proC with a hairpin in 5'UTR [BBa_K1741013]

proD [BBa_K1741014]


Teamuamprocdiffsugarsgraph.png

Teamuampoznanprochairpindiffsuagrsgraph.png

Teamuampoznanproddiffsugarsgraph.png

The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.

We observed that proD is up to three times stronger than proC. We need to enhance the hairpin in proC with 5'UTR hairpin to stop the translation in 23° C.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101
    Illegal SapI.rc site found at 232


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