Part:BBa_K1741002
sfGFP under a short arabinose promoter s2 without O1 and O2
This, shortened version of araBAD promoter (BBa_K1481002) does not contain O1 and O2 regions, but still contains the CRP binding site.
Design
Legend
AraWT - [BBa_K1481002]
Arashort1 - [BBa_1471000]
Arashort2 - [BBa_1471002]
For the design of our modified arabinose promoters we used the BioBrick created by our fellow iGEM team - PoznanBioInf. Their promoter was used as a control for out other arabinose promoters. We shortened the sequence of the original promoter by removing lengthy AraC part (we called that construct Arashort1) or removing AraC, O1 and O2 regions (we called that construct Arashort2).
Results
Legend
AraWT - [BBa_K1481002]
Arashort1 - [BBa_1471000]
Arashort2 - [BBa_1471002]
The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.
All of our arabinose promoters are induced by the arabinose and repressed by the glucose. Arashort1 works better than AraWT. On the other hand, Arashort2 is slightly weaker when compared to Arashort1, but still stronger than AraWT. Probably O1 and O2 regions that were removed in Arashort2 are necessary for the best promoter's performance.
We also checked the tightness of our promoters.
All of our promoters are induced only by their respective sugars with a small exception of xylose promoters being slightly induced by arabinose and arabinose promoters being slightly induced by xylose. All of our promoters are repressed by glucose.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 54
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 170
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