Generator

Part:BBa_K1741007

Designed by: Marta Żardecka   Group: iGEM15_UAM_Poznan   (2015-09-17)
Revision as of 05:50, 27 September 2015 by Daria (Talk | contribs)

sfGFP under xylose promoter xylA (xylWT)

SfGFP under xylose promoter xylA (wild type).

Characteristics and Results

Legend:

XylWT (XylA) [BBa_K1741007]

XylA1 [BBa_K1741008]

XylS [BBa_K1741009]

XylF [BBa_K1741010]

Teamuampoznanxyloseod600graph.png

Teamuampoznanxyloselbgraph.png

Teamuampoznanxylosem9graph.png

The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.

All our xylose promoters are induced by xyolse and repressed by glucose. Genes involved in catabolism of xylose in E.coli are organized into two transcription units which are driven by two promoters - XylA and XylF. In XylA1 we have modified the 5'UTR of the XylWT promoter so it was similar to that of proD [BBa_K1741014]a strong constitutive promoter in order to increase the expression of a protein under our promoter. The next step was to shorten the promoter by remaning its XylF part while keeping thw modified UTR. Finally we tested the XylF promoter upstream of sfGFP. XylS is the strongest xylose induced promoter Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 153
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 408


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