Generator

Part:BBa_K1741004

Designed by: Marta Żardecka   Group: iGEM15_UAM_Poznan   (2015-09-17)
Revision as of 04:40, 27 September 2015 by Michal.Kopa (Talk | contribs) (→‎Results)

sfGFP under melibiose promoter (improved 5'UTR)

Twice as high expression (comparing to BBa_K1741003) has been obtained by 5’UTR editing – removal of potential secondary structure which involved RBS and a better positioning of RBS – 7 nt upstream AUG start codon. The modified 5’UTR results in about twice higher expression upon melibiose addition to the M9 or 2xLB medium. The promoter is still weaker than araBAD or rhaBAD, so can be the promoter of choice if lower expression level is necessary for efficient folding or secretion of a recombinant protein.

Results



Legend:
MelS BBa_K1741004
MelWT BBa_K1741003

picture Teamuampoznanmelibioselbgraph.png

picture Teamuampoznanmelibiosem9graph.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 207


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