Composite

Part:BBa_K1800000:Experience

Designed by: Laura Lee Irvin   Group: iGEM15_Georgia_State   (2015-08-25)
Revision as of 23:03, 26 September 2015 by Jsiemer (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.


The 2015 GSU iGEM team was able to successfully express and characterize the mambalgin protein in E. coli. After inducing with IPTG overnight, the cell cultures were centrifuged and disrupted with a French press. Mambalgin was isolated using affinity chromatography, utilizing the 6x His tag in the construct. A SDS-PAGE was done, followed by a Ponceau S. and coomassie stain to visualize the proteins. A band indicating a protein of ~9 kda – the size of the mambalgin for E. coli construct – was seen in the eluate fraction <img src="MAMBA.E.coli_Western_blot.THISONE.jpg">

Applications of BBa_K1800000

User Reviews

UNIQd4480cf10c6fcbe9-partinfo-00000000-QINU UNIQd4480cf10c6fcbe9-partinfo-00000001-QINU