Coding

Part:BBa_K1783004:Design

Designed by: Juhye Kang, Kara Huie, Iowis Zhu   Group: iGEM15_UMaryland   (2015-08-14)
Revision as of 04:48, 26 September 2015 by Kabtahi (Talk | contribs) (→‎Design Notes)


Epsilon Cyclase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 91
    Illegal BamHI site found at 1398
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 223
    Illegal AgeI site found at 1030
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1105


Design Notes

This BioBrick was created by DNA synthesis through IDT. The gBlock of this part was cloned into a linearized pSB1C3 backbone with Gibson assembly. Was codon optimized for E. coli production using IDT Codon Optimizer.

Source

From genome of Arabidopsis thaliana.

References