Measurement

Part:BBa_K1783003

Designed by: Iowis Zhu   Group: iGEM15_UMaryland   (2015-08-14)
Revision as of 03:07, 26 September 2015 by Kabtahi (Talk | contribs)

Unstable RFP under Hok/Sok Regulation

Produces unstable RFP under a constitutive promoter. This construct carries the hok/sok toxin/antitoxin (TA) plasmid maintenance system and was used to measure the ability of this alternative plasmid maintenance system to keep plasmids within the cell without the use of antibiotics.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 587
    Illegal NheI site found at 610
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1199
    Illegal AgeI site found at 1311
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None