Part:BBa_K1618021

Plac-THL-CRO-BHBD-BCD-EFTb-EFTa

K1618021 contains the coding sequences for the Thiolase,Crotonase, β-hydroxybutyryl-CoA Dehydrogenase,Butyryl-CoA Dehydrogenase,Electron Transfer Protein β and Electron Transfer Protein α from Coprococcus sp. (L2-50 DSM),under control of the LacI promoter. This construct builds the butyrate pathway (Figure 1) that converts acetyl-CoA into Butyry-CoA ¹. At the last moment we realised that there was an EcoRI site on the gene sequence so we're working to fix this as soon as possible.

1. Louis P., Petra L., Pauline Y., Grietje H., and Flint H.J., 2010, Diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-CoA:acetate CoA-transferase gene , Environmental Microbiology, 12(2) p. 304–14..

Figure 1: The butyrate biosynthetic pathway.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 229
  Illegal EcoRI site found at 3159
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 229
  Illegal EcoRI site found at 3159
  Illegal NheI site found at 1167
  Illegal NotI site found at 235
  Illegal NotI site found at 3841
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 229
  Illegal EcoRI site found at 3159
  Illegal BglII site found at 1415
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 229
  Illegal EcoRI site found at 3159
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 229
  Illegal EcoRI site found at 3159
  Illegal XbaI site found at 244
  Illegal AgeI site found at 1338
  Illegal AgeI site found at 3354
  
• 1000
  COMPATIBLE WITH RFC[1000]