Composite

Part:BBa_K1618014

Designed by: Kieran Rustage   Group: iGEM15_NRP-UEA-Norwich   (2015-08-28)
Revision as of 02:27, 26 September 2015 by PilarCorMo (Talk | contribs)

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Plac-THL

K1618014 contains the coding sequence for the enzyme thiolase of the butyrate biosynthetic pathways (Figure 1) from Coprococcus sp. (L2-50 DSM 16842) under control of the LacI promoter. Thiolase catalyzes the Conversion of Acetyl-CoA to Acetoacetyl-CoA 1.

1. Louis P., Petra L., Pauline Y., Grietje H., and Flint H.J., 2010, Diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-CoA:acetate CoA-transferase gene , Environmental Microbiology, 12(2) p. 304–14.

NRP-UEA-Norwich-Flavs-Thing.png

Figure 1: The butyrate biosynthetic pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1145
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1393
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1316
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None