Part:BBa_K392001:Experience
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Figure 1 shows the results for the pENO2-RFP construct. Surprisingly, the RFP is localized only at the membrane, even though RFP without membrane target sequence was used.
Figure 1: Fluorescence pictures of cells expressing pENO2-RFP. The RFP is localising to the plasma membrane on the cells.
We therefore sequenced the whole construct and saw that the sequence of the promoter did not correspond to the sequence given in the registry. The sequence we found was:
TTATTATTTGTATAGTTCATCCATGCCATGTGTAATCCCAGCAGCTGTTACAAACTCAAGAAGGACCATGTGGTCTCTCTTTTCGTTGGGATCTTTCGAAAGGGCAGATTGTGTGGACAGGTAATGGTTGTCTGGTAAAnGGACAGGGCCATCGCCAATTGGAGTATTTTGTTGATAATGGTCTGCTAGTTGAACGCTTCCATCTTCAATGTTGTGTCTAATTTTGAAGTTAACTTTGATTCCATTCTTTTGTTTGTCTGCCATGATGTATACATTGTGTGAGTTATAGTTGTATTCCAATTTGTGTCCAAgAATGTTTCCATCTTCTTTAAAATCAATACCTTTTAACTCGATTCTATTAaCAAGGGTATCACCTTCAAACTTGACTTCAGCACGTGTCTTGTAGTTCC
UNIQea4acf17e843a661-partinfo-00000000-QINU UNIQea4acf17e843a661-partinfo-00000001-QINU