Part:BBa_K1613023

B0015:I721001

This part is a ligation of B0015 and I721001---the former fragments of K1613017. The description below is from the information of K1613017.

This part is desinged to proceed lead detection in e.coli. When lead ions (Pb2+) enter the e.coli, they will combine with the protein PbrR and then form a dimer. The lead promoter can be controlled by the dimer to further trigger the RFP. The idea of this design came from Brown 2007 igem team, also working on lead detection. We combined I721001 and I721002 from their teams with a constitutive promoter J23102 and RFP to make them function.

Here are our product design: 1. The tube is divided into the left side (red) and the right side (blue) by a partition. The red side is a straw for effectiveness test. Press the red straw so that the test copper ion is added into microcapsules. Green fluorescence means the E.coli survives; if not, we can know that the product is unavailable. The blue side is a tube for piercing. Press the blue straw to pierce chitosan so that the oil can go into the layer of microcapsules. 2. Microcapsule (micro-powder-like): Microcapsule is the most important part of this product, which is made by freeze drying so that the water is reduced. As long as it exposes to any substances such as heavy metals, benzopyrene and aflatoxin, green fluorescence will soon express and from that we know there are toxins in the edible oil. Without tough inspection process, we can easily test the oil samples with very low cost. 3. Chitosan: both layers of chitosan are exceedingly easy to dissolve in acetic acid, which has a big effect in attach to cooper(II) ion. 4. The bottom layer of the tube is sodium percarbonate, an oxidizing agent. Sodium percarbonate is an ingredient in a number of home and laundry cleaning products, including non-chlorine bleach products such as OxiClean, Tide laundry detergent, and Vanish. Dissolved in water, it yields a mixture of hydrogen peroxide (which eventually decomposes to water and oxygen) and sodium carbonate ("soda ash").

Whether lead can enter e.coli or not: By using gold nanoparticles(AU-NPs), we measured the lead absorption of e.coli. The results showed that the longer e.coli put in Pb2+ or the higher Pb2+ concentration, the more lead entered the e.coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 217