Regulatory

Part:BBa_K392001:Experience

Designed by: Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda   Group: iGEM10_Osaka   (2010-10-10)
Revision as of 02:11, 26 September 2015 by KSab (Talk | contribs)

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Applications of BBa_K392001

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Figure 1 shows the results for the pENO2-RFP construct. Surprisingly, the RFP is localized only at the membrane, even though RFP without membrane target sequence was used. We therefore sequenced the whole construct and saw that the sequence of the promoter did not correspond to the sequence given in the registry.

Team Tuebingen pENO2-RFP.png


Figure 1: Fluorescence pictures of cells expressing pENO2-RFP. The RFP is localising to the plasma membrane on the cells.

UNIQd039316e821a1335-partinfo-00000000-QINU UNIQd039316e821a1335-partinfo-00000001-QINU