Coding

Part:BBa_K1680021:Design

Designed by: Nicolai von Kuegelgen   Group: iGEM15_Tuebingen   (2015-09-17)
Revision as of 01:31, 26 September 2015 by KatharinaS (Talk | contribs) (→‎Design Notes)


Dronpa caged Cre with NLS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 298
    Illegal BglII site found at 2032
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1154
    Illegal SapI site found at 704
    Illegal SapI site found at 1751


Design Notes

3A Assembly using RFC25

Source

Composite part of part:BBa_K1680006, part:BBa_K1680007 and part:BBa_K16800017 .

References

Zhou, X. X., Chung, H. K., Lam, A. J., & Lin, M. Z. (2012). Optical control of protein activity by fluorescent protein domains. Science, 338(6108), 810-814. doi: 10.1126/science.1226854