Plasmid_Backbone
Part:BBa_K1680026
Designed by: Katharina Sporbeck Group: iGEM15_Tuebingen (2015-09-18)
pETUE
E. coli expression plasmid. Overexpression is IPTG inducible in the RFC25 Standard. It also encodes for a 6xHis-tag with a Thrombin cleavage site between coding region and 6xHis-tag, so that the His-tag can easily be cleaved off. Vector can be linearized with PstI/XmaI, and parts can be cloned into it with PstI/NgoMIV. Ampicillin resistance.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal PstI site found at 133 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal PstI site found at 133 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal PstI site found at 133 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal PstI site found at 133
Illegal NgoMIV site found at 892
Illegal NgoMIV site found at 3053
Illegal NgoMIV site found at 3213
Illegal AgeI site found at 3270 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 374
Illegal BsaI.rc site found at 1477
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//plasmid
//plasmid/expression
//plasmid/expression/t7
//plasmidbackbone/copynumber/high
//plasmidbackbone/expression/inducible
//plasmid
//plasmid/expression
//plasmid/expression/t7
//plasmidbackbone/copynumber/high
//plasmidbackbone/expression/inducible
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