Regulatory

Part:BBa_K563004:Experience

Designed by: Boxuan Zeng   Group: iGEM11_Tianjin   (2011-10-05)
Revision as of 20:53, 25 September 2015 by David.jullesson (Talk | contribs)

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Please enter how you used this part and how it worked out.

Applications of BBa_K563004

User Reviews

UNIQac235b3171eaf351-partinfo-00000000-QINU UNIQac235b3171eaf351-partinfo-00000001-QINU

UNIQac235b3171eaf351-partinfo-00000002-QINU

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UCSF-UCB iGEM 2014

We used pTEF1 in pSV606 (S. cerevisiae) yeast strains to create a constitutive circuit for our project, and it performed consistently as a constitutive promoter. We characterized pTEF1 alongside some of its mutants (m3, m6, m7, and m10), as seen in the following graph. If one would like to optimize expressiveness, pTEFm7 was found to have a higher level of constitutiveness than pTEF1, but pTEF1 is still a reliable promoter.

140630 pTEF1 GFP ROUND 1.png

The mutants were selected from a 2006 paper by Elke Nevoigt and Jessica Kohnke: http://www.ncbi.nlm.nih.gov/pubmed/16885275.

pTEF1 is also listed as parts BBa_K431008 and BBa_K319003.


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Chalmers 2015

Additional improvement added by Author: John Hellgren Summary: The high expression pTEF1 promoter connected to pSUC2 promoter. Can be used for induced high expression of any coding part in Saccharomyces cerevisiae at low ATP levels. Improves the function of pTEF1 by adding repression at high levels of ATP and induction at low levels of ATP. pTEF1-pSUC2