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Composite

Part:BBa_K1602044

Designed by: Christian Sator,Stefan Zens, Max Zander, Benedikt Spannenkrebs, Sebastian Jaeger   Group: iGEM15_TU_Darmstadt   (2015-09-16)
Revision as of 09:15, 25 September 2015 by CSator (Talk | contribs)

RRL3H

Composite part consisting of a constitutive promoter (BBa_J23100), Lock3 (BBa_J01080) and hokD (BBa_K1497008). It belongs to a two-part killswitch-system for E.coli utillizing a riboregulator for posttransciptional regulation of hokD. Upon transcription the sequence of Lock3 forms a hairpin-secondary-structure masking the ribosome binding site (RBS) and therefore prevents translation of the following hokD-sequence. If the corresponding trans-activating RNA-sequence (taRNA) (Key3 BBa_J01086) is present the two sequences form a RNA-RNA-complex. This leads to a helix shift and the release of the RBS enabling the expression of hokD resulting in cell death.

Figure 1: Interaction of taRNA and crRNA leads to expression of hokD.
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Functional Parameters

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
None