Composite

Part:BBa_K1582016:Design

Designed by: Dongqi Bao   Group: iGEM15_Tianjin   (2015-09-08)
Revision as of 02:54, 25 September 2015 by Sherry222 (Talk | contribs) (→‎Design Notes)

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LC+sJanus Fusion Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1141
    Illegal AgeI site found at 600
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1150


Design Notes

Linker:
GGTGGTGGTGGTTCTGGTGGTGGTGGTTCT
Codon optimized to:
ACCTACTCTGACAACCGTTACGGTGGTACCTGCGACCCGGACGGTTGCGACTGGAACCCGTACCGTCTGGGTAAC

Source

References