Coding
KERA

Part:BBa_K1717171

Designed by: Our Lady of the Snows Catholic Academy 2015   Group: iGEM15_OLS_Canmore_AB_CA   (2015-09-13)
Revision as of 01:33, 25 September 2015 by Jpuurunen (Talk | contribs)

Protein coding sequence for KeratinaseA

Keratinases are proteolytic enzymes which break the disulphide bonds in various keratin substrates. KERA is a basic part comprised from a synthesized KERA protein coding sequence, optimized for expression in E. coli and most active in degrading keratin in feathers. This gene, when expressed in cells, can be tested for activity through looking for zones of clearing on skim milk agar plates where cells are growing, as well as by looking at degradation of keratin-containing substrates (feathers, hair, chemically synthesized keratin, etc.) when grown with cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 130
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 391
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 761
    Illegal SapI site found at 1064


[edit]
Categories
Parameters
None