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Notebook

Ligation of Gblocks Holin-Endolysin into pDawn

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Tube	H/E
Water	4,6 µL
T4 ligase Buffer	1 µL
pDawn	1,4 µL
Plasmid	2,5 µL
T4 ligase	0,5 µL

Results

Bacteria expressing pDawn-HE were prepared in a pre-culture until DO600 reached a value between 0,6 and 0,8. Then, one condition was illuminated with white-light and incubated at 37°C, another one was kept in the dark and incubated at 37°C and the last condition was illuminated with white-light and incubated at room temperature. The value of DO was taken regularly to observe the kinetic of growth under each condition.

In absence of light, and at 37°C, bacteria growth was normal but light illumination was enough to slow-down the growth of bacteria. Incubation at room temperature with white-light illumination allowed better folding of HE and had a stronger effect on the growth of bacteria.

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