Generator

Part:BBa_K1859020:Experience

Designed by: Yusuke Banno   Group: iGEM15_Gifu   (2015-09-09)
Revision as of 09:35, 22 September 2015 by Nomuken (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1859020

User Reviews

UNIQ0e188aa2faf826a8-partinfo-00000000-QINU UNIQ0e188aa2faf826a8-partinfo-00000001-QINU

We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane E shows this sample.


left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay

There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.