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Part:BBa_K1859022:Experience

Designed by: Yusuke Banno   Group: iGEM15_Gifu   (2015-09-10)
Revision as of 09:21, 22 September 2015 by Kozakai (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

===Applications of BBa_K1859022===

We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane G shows this sample.


left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay

There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.

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