Generator

Part:BBa_K1859020:Experience

Designed by: Yusuke Banno   Group: iGEM15_Gifu   (2015-09-09)
Revision as of 09:15, 22 September 2015 by Kozakai (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

===Applications of BBa_K1859020===

We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane E shows this sample.


left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay

There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.
.

User Reviews

UNIQ60eeed61c87b0c54-partinfo-00000001-QINU UNIQ60eeed61c87b0c54-partinfo-00000002-QINU